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KMID : 0379119820100020067
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Korean Journal of Mycology
1982 Volume.10 No. 2 p.67 ~ p.73
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Studies on the Isolation , Purification and Characterization of a Cx Enzyme Produced by Pyricularia oryzae , C-7+t
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Abstract
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The (NH₄)₂ SO₄, (70%) treated crude enzymes from the culture filtrates of the C-7^(+1) strain of Pyricularia oryzae which was grown on 2% CMC (carboxymethyl cellulose) for 8 days at 28℃, were chromatographied on Sephadex G-150 and DEAE-Sephadex A-25 columns. From the chromatography, three fractions of CMCase(C_x) was examined using Na-CMC as substrate. The C_x enzyme activity was optimal at pH 6.0 and 40℃, stable up to 40℃. The values of Km and Vmax of the enzyme were 2.8 × 10 mM and 5.9m moles/hour, respectively. The molecular weight determined by Sephadex G-150 column chromatography was around 80,000. Approximately sevenfold purified C_x enzyme gave a single protein band on the polyacrylamide gel electrophoresis.
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